广西地方鸡微卫星位点LEI0258多态性研究

摘要

通过微卫星位点LEI0258基因分型和基因测序,分析6个广西地方鸡品种的遗传变异并构建中介网络图,研究广西地方鸡的遗传多样性与分子进化.结果表明,120份样品经基因分型,检测到38个等位基因,长度为181～507 bp,等位基因205出现的频率最高(0.125),其次是275(0.113)、310(0.100)和251(0.067).广西地方鸡保持着较高的遗传多样性水平,观察杂合度、期望杂合度和多态信息含量分别为0.800、0.943和0.936.经测序鉴定出39个等位基因,其中9个为首次发现.11个等位基因与已知的21种血清型相对应.基于侧翼序列变异信息的中介网络图显示,39个等位基因分为3个进化枝,红原鸡的部分基因存在于这些进化枝中,提示红原鸡可能是广西地方鸡的主要祖先.%In order to uncover the genetic diversity and molecular evolution of indigenous chicken of Guangxi province,120 blood samples from 6 breeds were used to microsatellite LEI0258 genotyping and sequencing,and the data were used to analyse genetic variation and construct median-joining network.A total of 38 alleles ranging from 181 bp to 507 bp were found by genotyping,with the allele frequency arrangement of 205 (0.125),275 (0.113),310 (0.100) and 251 (0.067).The LEI0258 showed high levels of polymorphism in Guangxi indigenous chickens,with 0.800,0.943 and 0.936 for observed heterozygosity,expected heterozygosity and polymorphic information content,respectively.Thirty-nine alleles were identified by DNA sequencing,and nine of which were newly found.Eleven alleles of LEI0258 were corresponding to 21 available serotypes of MHC.The median-joining network,which was based on the SNPs and indels found within the flanking sequences,39 alleles were classified into three clusters,and partial of those were also found in Red junglefowl.Our results pointed out the Red junglefowl origin for Guangxi indigenous chickens.