GeneFinder^（TM）核酸染料对玉米花粉管通道导入路径的研究

摘要

Pollen tube pathway was direct translation technology which useed plant antilogous egg cells or fertilized egg cells as the transformation object.It was a distinctive transgenic technology. This test added GeneFinderTM nucleic acid dye to exogenous DNA, after introduction exogenous DNA,ultraviolet imager showed the light different comparison of corn filament at different stage, which could observe the deformation path of exogenous DNA at different stage, so this way provided another new testing way to study introduction path of exogenous DNA.%花粉管通道法是一种借助于植物自身卵细胞或受精卵为转化对象的直接转化技术，是一种颇具特色的转基因技术。本试验将GeneFinder^（TM）核酸染料加入外源DNA中，通过紫外成像仪对外源DNA导入后不同时期玉米花丝在亮度上的对比差异来观察外源DNA不同时间段所通过的路径变化，因而为研究外源DNA导入路径提供了一种新的试验方法。