小米中的黄色素是一类类胡萝卜素化合物。作为杂粮食品中的重要功能性成分，其成分和含量分析受到极大关注。建立了小米中黄色素物质的反相高效液相色谱分析方法。色谱分离柱采用YMCTM Carotenoid S-5（250×4.6 mm ID，5μm），流动相采用三元溶剂梯度洗脱模式，样品中目标组分采用甲基叔丁基醚（MTBE）超声波辅助萃取处理。色谱分离获得了较好的选择性，各黄色素组分17 min内完成基线分离，并在0.5~50.0μg ·mL-1浓度范围内呈现良好的线性关系，方法叶黄素、玉米黄素和β-隐黄素的检测限分别为0.3，0.4和0.4μg ·mL-1。该方法样品前处理简便，重现性好，结果准确。%Yellow pigment in millet was a group of carotinoid compounds. As the important functional ingredients of cereal foodstuff, the component analysis of yellow pigment in millet was attracted extensive attention. In this paper,the determination method of yellow pigment in millet was established with reversed-phase high performance liquid chromatography. A YMC TM Carotenoid S-5 (250*4.6 mm ID,5 μm)column was used for the separation,and the mobile phase was subjected to gradient elution with triple-solvent. Samples were pretreated by ultrasonic wave assisted-extraction with methyl tert-butyl ether(MTBE).The separation obtained the highly sensitivity and selectivity. The separation was achieved with in 17 min. The calibration curve was linear in the mass concentration range of 0.5-50.0 μg·mL-1,and the LOD of lutein,zeaxanthin and β-cryptoxanthin were 0.3,0.4 and 0.4 μg·mL-1, respectively. The method was simple,accurate and reproducible.
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