甜高粱蔗糖转运蛋白SUT1基因表达载体的构建及转化农杆菌

摘要

为了获得甜高粱蔗糖转运蛋白SUT1基因的植物表达载体。采用PCR方法对甜高粱SUT1基因全长cDNA开放阅读框进行扩增，并将其与植物表达载体UBI-1300-GFP进行连接，连接后转化农杆菌EHA105。实验结果表明，成功克隆了SUT1基因开放阅读框，并将含有目的基因的重组表达载体转化到了农杆菌中。这将为进一步研究甜高粱SUT1基因的功能具有重要意义。%In order to construct plant expression vector of sweet sorghum sucrose transporter SUT1 gene,the full-length cDNA of sorghum SUT1 gene open reading frame was amplified by using PCR method,and PCR product was cloned into the plant expression vector UBI-1300-GFP,and the connection was transformed into Agrobacterium EHA105 competent cells. The results showed that the open reading frame of SUT1 gene was amplified successfully,and the recombinant expression vector that contained the objective gene was transformed into Agrobacterium EHA105. This would have an important foundation for further gene study of sweet sorghum SUT1 gene.