甩片改良抗酸法与 FQ-PCR 技术在脑脊液抗酸菌检查中的应用分析

摘要

Objective To evaluate united test significance in the diagnosis of tuberculous meningitis (TBM)with modified acid-fast staining method of cerebrospinal fluid and real-time fluorescence quantitative polymerase chain reaction (FQ-PCR).Methods Cerebrospinal fluid samples from 81 patients with TBM were detected for Mycobacterium tuberculosis respectively with centrifugal modified acid-fast staining method and real-time FQ-PCR.Results The positive rate of modified acid-fast staining 66.7%(54/81)was significantly higher than that of FQ-PCR 50.6%(41/81),there was significant difference between the two methods(P <0.05).Conclusion Modified acid-fast staining method is significantly better than FQ-PCR in the diagnosis of TBM.%目的：评价脑脊液改良抗酸染色法与实时荧光定量聚合酶链反应（fluorescence quantitative polymerase chain reaction，FQ-PCR）法诊断结核性脑膜炎（tuberculous meningitis，TBM）的意义。方法 TBM 患者81例，采集脑脊液检测标本，分别采用离心甩片改良抗酸染色法、实时 FQ-PCR 法检测抗酸杆菌。结果81例改良抗酸染色法阳性率66．7％（54／81）高于 FQ-PCR 方法阳性率50．6％（41／81），差异有统计学意义（P ＜0．05）。结论在诊断 TBM 方面，改良抗酸染色方法显著优于 FQ-PCR 方法。