钛螺纹钉表面镧羟基磷灰石复合涂层骨结合的实验研究

摘要

Objective To investigate the effect of lanthanum-hydroxyapatite composite coating on osseointegration of the bone around titanium screw.Methods Nine New Zealand white rabbits were divided into two groups randomly(6 as in experimental grap and 3 in control group).After removing the 4 upper and lower incisors, the sockets were filled with xenogeneic freeze-dried bone.Then in experimental group, the titanium screw with lan-thanum-hydroxyapatite composite coating was implanted into socket immediately.In control group, the titanium screw with hydroxyapatite coating was taken the same procedure as in experimental group.The animals were executed respectively at 4 th, 8 th and 12 th week after operation, and the intact maxillary and mandibulary were harvested to be observed, and tested by gross observation, bone density analysis, torque test, and histological observation.Results In the two groups, the average gray values of the new bone near the titanium screw and the torque peak values of titanium screw were gradually increased with the time extension.And at the same time point of 4 th, 8 th, 12 th week after operation, the average gray values of the new bone near the titanium screw and the torque peak values of titanium screw in experimental group were higher than those of control group(P<0.05).The regenerated osseous tissue in experimental group shows an earlier formation and higher maturity of the lamellar bone than that in control group.Conclusion Under the condition of immediate implantation, hydroxyapatite coating and lanthanum hydroxyapatite composite coating can be used to obtain good osseointegration and bone repair of titanium screw and surrounding bone tissue.However, the lanthanum hydroxyapatite composited coating has better biological stability and biocompatibility, which can promote the osseointegration of titanium screw.%目的 探讨镧羟基磷灰石复合涂层在种植体周围骨结合中的作用.方法 9只新西兰大白兔随机分为实验组(n=6)与对照组(n=3),分别拔除上下4颗切牙,在所有拔牙窝下段充填入适量异种冻干骨后,实验组拔牙窝内即刻植入镧羟基磷灰石复合涂层的钛螺纹钉,对照组拔牙窝内则即刻植入羟基磷灰石涂层的钛螺纹钉.术后4、8、12周分别处死实验动物(实验组2只,对照组1只)取完整上下颌骨标本,进行大体观察、扭矩实验测试、X线片检查、骨密度分析和组织学观察.结果 随时间延长, 2组钛螺纹钉周围新生周骨组织平均灰度值和钛螺纹钉最大扭矩值均逐渐增高,且术后4、8、12周实验组钛螺纹钉周围新生周骨组织平均灰度值和钛螺纹钉最大扭矩值高于对照组(P<0.05).实验组板层新骨生成速度更快,新生骨成熟度更高.结论 在即刻种植条件下,羟基磷灰石涂层与镧羟基磷灰石复合涂层均可获得钛螺纹钉与周围骨组织较好的骨结合和骨修复,但镧羟基磷灰石复合涂层有更好的生物稳定性和生物相容性,能促进钛螺纹钉周围的骨结合.