The primary cultured endometrial cells in mice were isolated and purified by trypso-gen-EDTA digestion method, cultured in vitro and induced inflammation by lipopolysaccha-ride(LPS). The morphology and traditional inflammation factors of the cells were measured as the development criteria of inflammation. The cells were treated with graded concentrations of LPS. The cells were collected in different periods, and the contents of IFN - γ mRNA were determined by RT-PCR. The results showed that 100 ng/mL LPS was the optimal concentration in inducing the inflammation of cultured endometrial cells. The expression levels of IFN-y in the inflammatory model group were significantly higher than those in control at the same period (F<0. 01), the inflammatory model of the endometrial cells was created successfully.%用0.25％胰酶-EDTA消化法分离培养小鼠子宫内膜细胞,用细菌脂多糖(LPS)诱导子宫内膜细胞炎症,以培养细胞的形态学和传统炎症指标作为炎症发生和发展的判断标准.以不同浓度的LPS作用于细胞,收集不同时段的细胞,用RT-PCR的方法测定细胞中IFN-γmRNA的表达丰度.结果表明,100 ng/mL LPS是体外培养的子宫内膜细胞诱导炎症的最适浓度.炎症模型组培养细胞中IFN-γ的丰度表达极显著于空白组(P＜0.01),表明炎症模型建立成功.
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