Curcumin is Effective in Improving Folliculogenesis Profile (Primary and Secondary Folicles) and Oocytes Quality In Vitro in Ectopic Endometriosis Mice (Mus Musculus) Models

摘要

Endometriosis is the presence of ectopic endometrial tissue, such as that outside the uterine cavity, which can cause chronic inflammatory reactions. Curcumin is one of traditional herbal medicines that is widely used. Some experiments have managed to find a mechanism to treat an ectopic endometriosis through the mechanism of suppression of several cytokines. This study was an experimental laboratory study, with four treatments and five replications, using female mice (Mus musculus) that had reached puberty. The study consisted of three phases: the first phase was the microscopic examination of in vitro oocytes quality, and the second was histopathological examination of folliculogenesis profile using Hematoxillin eosin staining. The results of this study showed significant difference between positive control group (P0) with treatment and negative control (KN) groups in folliculogenesis profile (p <0.05). Examination of in vitro oocytes quality showed significant difference between positive control group with treatment group and negative control group (p <0.05). Curcumin has several roles in cytokines modulation in ectopic endometriosis mouse models. Curcumin may reduce the occurrence of apoptosis of granulosa cells so that it may directly improve oocyte quality and folliculogenesis profile (tersier follicel and de graf). In conclusion, curcumin effectively overcomes fertility problems through the mechanism of cytokines modulation that plays a role in some cases of endometriosis such as: TNF-alpha, NF-kB and COX-2. Curcumin may improve folliculogenesis profile and in vitro oocytes quality.