鉴于温度对产甲烷过程稳定运行的重要性,采用间歇培养方式研究了产甲烷过程受温度降低的影响,以及初始碳源对温度降低后产甲烷效能的影响.结果表明,产甲烷菌在25℃条件下,产甲烷效能有不同程度的下降,累计产甲烷量降低了25.2％,比产甲烷速率降低了21.1％,底物利用率都降低了15％左右.产甲烷菌在初始甲酸钠体积分数5％ ～40％、乙酸钠浓度0.5～4.0 mol/L和甲醇体积分数35％～70％时都可以产甲烷,其累计产甲烷量随着底物含量的增大而先增大后减小.在甲酸钠体积分数30％和乙酸钠浓度3 mol/L时,累计产甲烷量达到最大,分别为2.75、2.84 L/L,累计产甲烷量分别提高了12.2％和15.9％.不同体积分数甲醇对其产甲烷量提高效果不明显,产甲烷效能与原来持平.%This paper studied the effect of lower temperature and initial substrate concentrations on methane production by methanogens at 25 ℃ and sodium concentrations 5% ~ 40% , sodium acetate concentrations 0.5~4. 0 mol/L, methanol concentration was 35% — 70%. The cumulative methane volume decreased 25.2% , specific methane production rate decreased 21. 1% , substrate utilization rate lower about 15%. The cumulative hydrogen volume and biomass increased and then decreased with increasing initial substrate concentrations. At sodium concentrations 30% and sodium acetate concentrations 3 mol/L, the cumulative methane production reached the maximum value of 2. 75 L/L and 2. 84 L/L, decreased 12.2% and 15. 9% respectively. The effect of different concentrations of methanol ruas not obvious.
展开▼
