手工刮治结合龈下喷砂处理根面对牙周膜细胞生长的影响及分子机制探究

摘要

目的：：研究手工刮治结合龈下喷砂处理根面对牙周膜细胞生长的影响及分子机制。方法：因重度牙周炎拔除的磨牙和前磨牙用于牙根片制备,根据菌斑处理方法不同分为手工刮治组和联合处理组,手工刮治组采用 Gracey 手工刮治器进行单纯刮治,联合处理组在单纯刮治后进行喷砂处理；另取健康且无龋的前磨牙和第三磨牙,培养牙周膜细胞(hPDLC)并接种在不同的牙根片上,测定 hPDLC 的细胞活力、细胞周期以及 STRO-1、CD146、OPG、RANKL 的表达量。结果：不同根片处理后6 h、12 h、24 h时,联合处理组 hPDLC 在450 nm 处的 OD 值显著高于手工刮治组,PI 值显著低于手工刮治组；不同根片处理后24 h 时,联合处理组 hPDLC 中 STRO-1、CD146、OPG 的 mRNA 含量和蛋白含量显著高于手工刮治组,RANKL 的 mRNA 含量和蛋白含量显著低于手工刮治组。结论：手工刮治结合龈下喷砂处理根面有利于牙周膜细胞的生长,增强细胞分化潜能以及调节 OPG/RANKL 是相关的分子机制。%Objective:To study the effect of hand scaling combined with subgingival sandblasting treatment of root sur-face on human periodontal ligament cell growth and the molecular mechanisms.Methods:Molars and premolars extracted due to severe periodontitis were used for root slice preparation and divided into hand scaling group and combined treatment group according to different plaque treatment methods,hand scaling group used Gracey hand scaler for simple scaling and combined treatment group received sandblasting treatment after simple scaling;healthy and noncarious premolars and third molars were collected,human periodontal ligament cells (hPDLC)were cultured and inoculated on different root slices,and the hPDLC cell viability,cell cycle as well as STRO-1,CD146,OPG and RANKL expression levels were determined.Results:6 h,12 h and 24 h after different root slice treatment,OD values of hPDLC at 450 nm of combined treatment group were significantly higher than those of hand scaling group,and PI values were significantly lower than those of hand scaling group;24 h after different root slice treatment,mRNA content and protein content of STRO-1,CD146 and OPG in hPDLC of combined treatment group were significantly higher than those of hand scaling group while mRNA content and protein content of RANKL were signifi-cantly lower than those of hand scaling group.Conclusions:Hand scaling combined with subgingival sandblasting treatment of root surface helps the human periodontal ligament cell growth and the related molecular mechanisms are enhancing cell differen-tiation potential and regulating OPG/RANKL.