超声剪切波速度与原发性肝癌病灶中癌基因、抑癌基因表达及血管新生因子含量的关系

摘要

目的:探讨超声剪切波速度(SWV)与原发性肝癌病灶中癌基因、抑癌基因表达及血管新生因子含量的关系.方法:收集在本院接受手术治疗的原发性肝癌患者100例作为观察组,另取同期在本院进行体检的健康人50例作为正常对照组.术前测定两组研究对象的超声SWV水平,并进一步将观察组分为高SWV组、低SWV组各50例.留取术中肿瘤组织标本,采用荧光定量PCR法测定其中癌基因、抑癌基因mRNA的表达量.采用酶联免疫吸附法测定观察组术前血清中血管新生因子的含量.结果:观察组的肝脏超声SWV水平显著高于正常对照组(P<0.05);高SWV组肿瘤组织中原癌基因CK、Ki67、Gly-3、Survivin、Pokemon mRNA的表达量高于低SWV组,抑癌基因Tg737、p16、p27、PTEN、runx3 mRNA的表达量低于低SWV组;血清中血管新生因子VEGF、MMP-9、IGF-1R的含量高于低SWV组(P<0.05).结论:原发性肝癌患者肝脏超声SWV水平增高,且SWV水平与患者癌基因、抑癌基因表达及血管新生因子含量直接相关.%Objective: To discuss the relationship of ultrasonic shear wave velocity (SWV) with oncogene and tumor suppressor gene expression in primary liver cancer lesions as well as angiogenesis factor contents.Methods: A total of 100 patients with primary liver cancer who underwent surgical treatment in our hospital between March 2014 and September 2016 were collected as observation group, and 50 healthy subjects who received physical examination in our hospital during the same period were collected as normal control group.The ultrasonic SWV levels of two groups of subjects were measured before the operation, and the observation group were further divided into high SWV group and low SWV group, 50 cases in each group.Intraoperative tumor tissue samples were kept, and fluorescence quantitative PCR was used to determine the mRNA expression of oncogenes and tumor suppressor genes.Enzyme-linked immunosorbent assay was used to determine serum contents of angiogenesis factors in observation group before operation.Results:: Hepatic ultrasonic SWV level in observation group was significantly higher than that in normal control group (P<0.05);proto-oncogene CK, Ki67, Gly-3, Survivin and Pokemon mRNA expression in tumor tissue of high SWV group were higher than those of low SWV group while tumor suppressor genes Tg737, p16, p27, PTEN and runx3 mRNA expression were lower than those of low SWV group;serum angiogenesis factors VEGF, MMP-9 and IGF-1R contents were higher than those in low SWV group (P<0.05).Conclusions: The hepatic ultrasonic SWV level increases in patients with primary liver cancer, and the SWV level is directly correlated with oncogene and tumor suppressor gene expression as well as angiogenesis factor contents.