杜仲补骨脂促进离体培养成骨细胞增殖的实验研究

摘要

目的:研究杜仲和补骨脂处理对离体培养成骨细胞增殖的影响.方法:取1日龄的SD大鼠、分离培养露骨中的成骨细胞并分为4组,A组用不含药物及血清的DMEM处理,B组用杜仲终浓度为10 μg/L的无血清DMEM处理,C组用补骨脂终浓度为10 μmol/L的无血清DMEM处理,D组用杜仲终浓度为10 μg/L及补骨脂终浓度为10 μmol/L的无血清DMEM处理.处理后24小时,检测细胞中凋亡基因和增殖基因的mRNA表达量.结果:B组、C组、D组细胞中Bax、Fas、FasL、HSG的mRNA表达量均显著低于A组,c-fos、c-jun、CyclinD1、Egr-1、NDRG1的mRNA表达量均显著高于A组;D组细胞中Bax、Fas、FasL、HSG的mRNA表达量均显著低于B组和C组,c-fos、c-jun、CyclinD1、Egr-1、NDRG1的mRNA表达量均显著高于B组和C组.结论:杜仲和补骨脂能够促进成骨细胞的增殖且两药联用具有协同作用.%Objective: To study the effect of eucommia and fructus psoraleae treatment on the proliferation of osteoblasts cultured in vitro.Methods: One-day-old SD rats were taken, the osteoblasts in exposed bone were separated, cultured and divided into 4 groups, group A were treated with DMEM without drugs or serum, group B were treated with serum-free DMEM containing eucommia with final concentration 10 μg/L, group C were treated with serum-free DMEM containing fructus psoraleae with final concentration 10 μmol/L, and group D were treated with serum-free DMEM containing eucommia with final concentration 10 μg/L and fructus psoraleae with final concentration 10 μmol/L.Twenty-four hours after treatment, the mRNA expression of apoptosis genes and proliferation genes in cells were detected.Results:: Bax, Fas, FasL and HSG mRNA expression in group B, group C and group D were significantly lower than those in group A while c-fos, c-jun, CyclinD1, Egr-1 and NDRG1 mRNA expression were significantly higher than those in group A;Bax, Fas, FasL and HSG mRNA expression in group D were significantly lower than those in group B and group C, and c-fos, c-jun, CyclinD1, Egr-1 and NDRG1 mRNA expression were significantly higher than those in group B and group C.Conclusions: Both eucommia and fructus psoraleae can promote the osteoblast proliferation, and the combination of the two drugs has synergistic effect.