Objective: To study the effect of preoperative intracavitary radiotherapy combined with neoadjuvant chemotherapy on apoptosis in IIa and IIb cervical cancer tissue.Methods: Patients with IIa and IIb locally advanced cervical cancer who received preoperative auxiliary therapy in our hospital between May 2013 and December 2016 were retrospectively analyzed and divided into adjuvant chemotherapy group, intracavitary radiotherapy group and combined treatment group according to the different preoperative adjuvant therapies.Cervical cancer tissues were collected respectively during biopsy (before treatment) and during surgical excision (after treatment) to extract RNA, and then fluorescence quantitative PCR kits were used to determine the expression of pro-apoptosis genes, anti-apoptosis genes and invasion genes.Results: After treatment, SARI, Rab26, FHIT and eIF4E3 expression in cervical cancer lesions of three groups were significantly higher than those before treatment while serum CA125, TSGF and SCC-Ag levels as well as MRP8, MRP14, p38MAPK, ERK1/2 and c-myc expression were significantly lower than those before treatment, and SARI, Rab26, FHIT and eIF4E3 expression in cervical lesions of combined treatment group were significantly higher than those of adjuvant chemotherapy group and intracavitary radiotherapy group while serum CA125, TSGF and SCC-Ag levels as well as MRP8, MRP14, p38MAPK, ERK1/2 and c-myc expression were significantly lower than those of adjuvant chemotherapy group and intracavitary radiotherapy group.Conclusions: Preoperative intracavitary radiotherapy combined with neoadjuvant chemotherapy can promote apoptosis in IIa and IIb cervical cancer tissue.%目的:研究术前腔内放疗联合新辅助化疗对IIa和IIb期宫颈癌组织中细胞凋亡的影响.方法:选择在我院接受术前辅助治疗的IIa和IIb期局部晚期宫颈癌患者进行回顾性分析,根据术前辅助治疗方案不同分为辅助化疗组、腔内放疗组、联合治疗组.活检时(治疗前)和手术切除时(治疗后)分别留取宫颈癌组织,抽提RNA后采用荧光定量PCR试剂盒测定促凋亡基因、凋亡抑制基因、侵袭基因的表达量.结果:治疗后,三组宫颈癌病灶内SARI、Rab26、FHIT、eIF4E3的表达量均显著高于治疗前,血清中CA125、TSGF、SCC-Ag的含量以及MRP8、MRP14、p38MAPK、ERK1/2、c-myc的表达量均显著低于治疗前且联合治疗组宫颈癌病灶内SARI、Rab26、FHIT、eIF4E3的表达量均显著高于辅助化疗组、腔内放疗组,血清中CA125、TSGF、SCC-Ag的含量以及MRP8、MRP14、p38MAPK、ERK1/2、c-myc的表达量均显著低于辅助化疗组、腔内放疗组.结论:术前腔内放疗联合新辅助化疗能够促进IIa和IIb期宫颈癌组织中的表达凋亡.
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