骨碎补提取物调节成骨细胞活性、增殖及相关基因表达的实验研究

摘要

目的:研究骨碎补提取物对成骨细胞活性、增殖及相关基因表达的影响.方法:取SD大鼠,分离并培养股骨内的成骨细胞,分为用0.02 g/mL骨碎补提取物处理的骨碎补组以及用不含血清培养基处理的对照组;处理后24、36、48 h时,采用CCK-8试剂盒测定成骨细胞的增殖活力,采用荧光定量PCR试剂盒测定成骨活性相关基因、增殖相关基因的mRNA表达量.结果:处理后24、36、48 h时,骨碎补组成骨细胞的增殖活力值显著高于对照组;骨碎补组成骨细胞中Runx2、OPN、OCN、ALP、OPG、PI3K、AKT、EKR1/2、CyclinD1、CDK2、CDK4、E2F的mRNA表达量显著高于对照组.结论:骨碎补提取物能够促进成骨细胞增殖、增加成骨细胞的成骨活性,同时通过PI3K/AKT、ERK1/2信号通路加速细胞周期的进程.%Objective: To study the effecof rhizomdrynariae extracon osteoblasviability and proliferation awell arelated gene expression.Methods: SD ratwere selected, and the osteoblastin the femuwere isolated, cultured and divided into the rhizomdrynariae group thawere treated with 0.02 g/mL rhizomdrynariae extracand the control group thawere treated with serum-free medium;24 h, 36 h and 48 h aftetreatment, CCK-8 kitwere used to determine osteoblasproliferation activity, and fluorescenquantitative PCkitwere used to determine the mRNexpression of osteogeniactivity-related geneand proliferation-related genes.Results: Twenty-fouhours, 36 h and 48 h aftetreatment, osteoblasproliferation activity of rhizomdrynariae group wasignificantly highethan thaof control group;Runx2, OPN, OCN, ALP, OPG, PI3K, AKT, EKR1/2, CyclinD1, CDK2, CDK4 and E2F mRNexpression in osteoblastof rhizomdrynariae group were significantly highethan those of control group.Conclusions: Rhizomdrynariae extraccan promote osteoblasproliferation, increase the osteogenetiactivity of osteoblastand also accelerate the cell cycle procesthrough the PI3K/AKand ERK1/2 signaling pathways.