虫草素调节肺癌细胞株 A549恶性生物学行为的实验研究

摘要

目的：：研究虫草素对肺癌细胞株 A549增殖、凋亡、侵袭相关分子表达量的影响.方法：培养肺癌细胞株 A549,用不同剂量虫草素(0、0．25、0．5、1．0、2．0、4．0 ng/mL)处理24 h 后,测定细胞中增殖、凋亡、侵袭相关分子的 mRNA 表达量.结果：0．25、0．5、1．0、2．0、4．0 ng/mL 虫草素处理后,Caspase-3、Caspase-8、NOX1、LATS1的 mRNA 表达量均显著高于0 ng/mL 虫草素,CyclinD1、Bcl-2、c-Myc、c-FLIP、TRAF6、N-cadherin、Vimentin 的 mRNA 表达量均显著低于0 ng/mL 虫草素；虫草素剂量越大, Caspase-3、Caspase-8、NOX1、LATS1的 mRNA 表达量越高,CyclinD1、Bcl-2、c-Myc、c-FLIP、TRAF6、N-cadherin、Vimentin 的 mRNA 表达量越低.结论：虫草素对肺癌细胞株 A549中促凋亡基因的表达具有促进作用,对促增殖和促侵袭基因的表达具有抑制作用.%[ABSTRACT]Objective:To study the effect of cordycepin on proliferation,apoptosis and invasion-related molecule expres-sion in lung cancer cell lines A549.Methods:Lung cancer cell lines A549 were cultured and treated with different doses of cordycepin (0,0.25,0.5,1.0,2.0 and 4.0 ng/mL)for 24 h,and then the proliferation,apoptosis and invasion-related mole-cule mRNA expression in cells were detected.Results:After 0.25,0.5,1.0,2.0 and 4.0 ng/mL cordycepin treatment, Caspase-3,Caspase-8,NOX1 and LATS1 mRNA expression were significantly higher than those after 0 ng/mL cordycepin treatment while CyclinD1,Bcl-2,c-Myc,c-FLIP,TRAF6,N-cadherin and Vimentin mRNA expression were significantly lower than those after 0 ng/mL cordycepin traetment;the greater the cordycepin dosage,the higher the Caspase-3,Caspase-8, NOX1 and LATS1 mRNA expression,and the lower the CyclinD1,Bcl-2,c-Myc,c-FLIP,TRAF6,N-cadherin and Vimentin mRNA expression.Conclusion:Cordycepin can promote pro-apoptosis gene expression and inhibit pro-proliferation and pro-in-vasion gene expression in lung cancer cell lines A549.