热射病模型大鼠凝血功能、炎症反应程度及脑组织内细胞凋亡的评估

摘要

[ABSTRACT]Objective:To study the blood coagulation function,degree of inflammation as well as apoptosis in brain tis-sue of rat models with heat stroke.Methods:Healthy male SD rats were selected as experimental animals and randomly divided into heat stroke group and control group,heat stroke group were raised under the condition of environment temperature of (40. 0±0.5)℃ and relative humidity of 60% to establish heat stroke models,the anus temperature reached 42.5℃,and the control group were conventionally raised;60 min and 120 min after model establishment,the blood coagulation function,serum in-flammatory factor levels as well as brain water content,the number of apoptotic cells and apoptotic molecule expression were determined respectively.Results:(1)blood biochemical indexes:APTT and PT of heat stroke group were longer than those of control group,and serum D-D,TNF-α,IL-1β and IL-6 levels were significantly higher than those of control group;(2)the brain tissue damage indexes:brain water content,the number of apoptotic cells,Caspase-3 expression and LC3-II/LC3-I ratio of heat stroke group were significantly higher than those of control group.Conclusion:There are blood coagulation dysfunction and systemic inflammation activation in heat stroke model rats,and the apoptosis in brain tissue increases obviously.%目的：：研究热射病模型大鼠的凝血功能、炎症反应程度及脑组织内细胞凋亡.方法：选择健康雄性 SD 大鼠作为实验动物，随机分为热射病组和对照组，热射病组在环境温度(40．0±0．5)℃、相对湿度60％的条件下建立热射病模型并使肛温达到42．5℃、对照组进行常规饲养；造模后60 min、120 min 时，分别测定凝血功能、血清炎性因子含量以及脑组织含水量、凋亡细胞数目、凋亡分子表达量.结果：(1)血液生化指标：热射病组大鼠的 APTT、PT 长于对照组，血清中 D-D、TNF-α、IL-1β、IL-6的含量均显著高于对照组；(2)脑组织损伤指标：热射病组大鼠的脑组织含水量、凋亡细胞数目、Caspase-3表达量以及LC3-II/LC3-I 比值均显著高于对照组.结论：热射病模型大鼠存在凝血功能障碍、全身炎症反应激活且脑组织中细胞凋亡明显增多.