丁苯酞对血管性痴呆患者血清中细胞因子、可溶性凋亡因子、抗氧化分子的影响

摘要

目的：：探讨丁苯酞对血管性痴呆患者血清中细胞因子、可溶性凋亡因子、抗氧化分子的影响.方法：收集本院收治的血管性痴呆患者86例作回顾性研究,按照双盲随机对照法分为观察组、对照组各43例.观察组在常规治疗基础上加入丁苯酞胶囊(0．2 g/次,3次/d)治疗,对照组仅接受常规治疗,均持续24周.治疗24周后,采用 ELISA 法测定两组的血清血管修复因子、可溶性凋亡因子含量,采用放射免疫法测定血清抗氧化分子含量.结果：治疗24周后,观察组的血清血管修复因子血红素氧化酶-1(HO-1)、血管生成素Ⅰ(Ang Ⅰ)、血管内皮生长因子(VEGF)含量高于对照组,基质金属蛋白酶-9(MMP-9)含量低于对照组,组间差异有统计学意义(P ＜0．05)；血清可溶性凋亡相关因子(sFas)、可溶性凋亡相关因子配体(sFasL)含量低于对照组；血清抗氧化分子超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化力(TAC)、过氧化氢酶(CAT)、谷胱甘肽硫转移酶(GST)、谷胱甘肽还原酶(GR)含量高于对照组.结论：丁苯酞可促使血管性痴呆患者的血管修复,同时抑制细胞凋亡进程、平衡机体氧化/抗氧化系统功能.%[ABSTRACT]Objective:To study the effects of butyl phthalide on serum cytokines,soluble apoptosis factors and antioxi-dant molecules in patients with vascular dementia.Methods:A total of 86 patients with vascular dementia admitted in our hos-pital were selected for retrospective study and divided into observation group and control group (n =43)according to double-blind randomized control.Observation group received butyl phthalide capsule (0.2 g/time,3 times/d)treatment on the basis of conventional treatment,control group received conventional treatment alone,and both lasted for 24 weeks.After 24 weeks of treatment,ELISA was used to determine serum levels of vascular repair factors and soluble apoptosis factors of two groups of patients,and radioimmunoassay was used to determine serum levels of antioxidant molecules.Results:After 24 weeks of treat-ment,serum vascular repair factors heme oxygenase-1 (HO-1),angiogenin Ⅰ (AngⅠ)and vascular endothelial growth factor (VEGF)levels of observation group were higher than those of control group,matrix metalloproteinase-9 (MMP-9)level was lower than that of control group,and differences between groups were statistically significant (P <0.05);serum soluble factor-related apoptosis (sFas)and soluble factor-related apoptosis ligand (sFasL)levels were lower than those of control group;ser-um antioxidant molecules superoxide dismutase (SOD),glutathione peroxidase (GSH-Px),total antioxidant capacity (TAC), catalase (CAT),glutathione S-transferase (GST)and glutathione reductase (GR)levels were higher than those of control group.Conclusion:Butyl phthalide can prompt vascular repair in patients with vascular dementia,and also inhibit apoptosis process and balance the body's oxidation/antioxidation system function.