重组人干扰素α2b雾化对手足口病患儿血清细胞因子及外周血免疫细胞的影响

摘要

Objective:To study the effects of recombinant human interferon α2b atomization on serum cytokines and pe-ripheral blood immune cells in children with HFMD.Methods:Children who were diagnosed with hand-foot-and-mouth disease in Tongji Hospital Tongji Medical College Huazhong University of Science & Technology between June 2014 and August 2017 were selected as the research subjects and randomly divided into the interferon α2b group who accepted human recombi-nant interferon α2b atomization combined with conventional symptomatic treatment and the control group who accepted conven-tional symptomatic treatment.The contents of cytokines in serum and the contents of immune cells in peripheral blood were de-termined before treatment as well as 3 days and 5 days after treatment.Results:3 days and 5 days after treatment,IL-6,IL-13,TNF-α,CRP,MCP-1,VCAM-1 and ICAM-1 contents in serum as well as the contents of CD19+CD24highCD27+B cells in peripheral blood of both groups of children with were significantly lower than those before treatment whereas the contents of CD3+CD4+T cells,CD3+CD8+T cells and CD16+CD56+NK cells in peripheral blood were higher than those before treatment (P<0.05),and IL-6,IL-13,TNF-α,CRP,MCP-1,VCAM-1 and ICAM-1 contents in serum as well as the contents of CD19+CD24highCD27+B cells in peripheral blood of interferon α2b group were significantly lower than those of control group whereas the contents of CD3+CD4+T cells,CD3+CD8+T cells and CD16+CD56+NK cells in peripheral blood were higher than those of control group(P<0.05).Conclusion:Recombinant human interferon α2b atomization treatment of HFMD can reduce the inflammatory response and improve the immune response.Key words:%目的:研究重组人干扰素α2b雾化对手足口病患儿血清细胞因子及外周血免疫细胞的影响.方法:选择在本院诊断为手足口病的患儿作为研究对象,随机分为接受重组人干扰素α2b雾化联合常规对症治疗的干扰素α2b组以及接受常规对症治疗的对照组.治疗前及治疗后3天、治疗后5天时,测定血清中细胞因子的含量及外周血中免疫细胞的含量.结果:治疗后3天及5天时,两组患儿血清中IL-6、IL-13、TNF-α、CRP、MCP-1、VCAM-1、ICAM-1的含量以及外周血中CD19+CD24highCD27+B细胞的含量均显著低于治疗前,外周血中CD3+CD4+T 细胞、CD3+CD8+T 细胞、CD16+CD56+N K细胞的含量均高于治疗前(P<0.05)且干扰素α2b组患儿血清中IL-6、IL-13、TNF-α、CRP、MCP-1、VCAM-1、ICAM-1的含量以及外周血中 CD19+CD24highCD27+B 细胞的含量均显著低于对照组,外周血中CD3+CD4+T细胞、CD3+CD8+T 细胞、CD16+CD56+NK 细胞的含量均高于对照组(P<0.05).结论:重组人干扰素α2b雾化治疗手足口病能够减轻炎症反应、改善免疫应答.