子痫前期产妇胎盘组织中metastin表达量及其对滋养细胞增殖、凋亡的影响

摘要

目的:探讨子痫前期产妇胎盘组织中metastin表达量及其对滋养细胞增殖、凋亡的影响.方法:收集2014年9月~2016年12月间在本院分娩的子痫前期产妇80例作为观察组,同期在本院进行分娩的正常产妇50例作为正常对照组.对比两组产妇胎盘组织中metastin蛋白的表达量,滋养细胞增殖、凋亡基因的表达量,采用Pearson检验评估子痫前期产妇胎盘组织中metastin表达量及其对滋养细胞增殖、凋亡的影响.结果:观察组产妇胎盘组织中metastin蛋白的表达量显著高于正常对照组产妇(P<0.05).观察组产妇胎盘组织中滋养细胞增殖基因Notch1、HLA-G mRNA的表达量低于正常对照组,PHLDA2 mRNA的表达量高于正常对照组产妇;凋亡基因bcl-2 mRNA的表达量低于正常对照组,bax、Fas、caspase-3 mRNA的表达量高于正常对照组产妇(P<0.05).经Pearson检验发现,子痫前期产妇胎盘组织中metastin蛋白表达量与滋养细胞增殖、凋亡基因表达量均直接相关.结论:子痫前期产妇胎盘组织中存在metastin蛋白异常高表达,且可直接影响滋养细胞的增殖、凋亡进程.%Objective:To investigate the metastin expression in the placenta tissue of preeclampsia and its effect on the trophoblast cell proliferation and apoptosis.Methods:80 puerperae with preeclampsia who gave birth in this hospital between September 2014 and December 2016 were collected as observation group and 50 normal puerperae who gave birth in the hospital during the same period were collected as normal control group.The metastin protein expression as well as the trophoblast cell proliferation and apoptosis gene expression in placenta tissue were compared between the two groups of puerperae,and Pearson test was used to evaluate the metastin expression in the placenta tissue of preeclampsia and its effect on the trophoblast cell pro-liferation and apoptosis.Results:metastin protein expression in the placenta tissue of observation group was significantly higher than that of normal control group(P<0.05).Trophoblast cell proliferation genes Notch1 and HLA-G mRNA expression in the placenta tissue of observation group were lower than those of normal control group whereas PHLDA 2 mRNA expression was higher than that of normal control group;apoptosis genes bcl-2 mRNA expression was lower than that of control group where-as bax,Fas and caspase-3 mRNA expression were higher than those of normal control group(P<0.05).Pearson test showed that metastin protein expression in the placenta tissue of puerperae with preeclampsia was directly correlated with the tropho-blast cell proliferation and apoptosis gene expression.Conclusion:There is abnormally high metastin protein expression in pla-centa tissue of puerperae with preeclampsia,and it can directly affect the proliferation and apoptosis process of trophoblast cells.