缺血性脑卒中患者外周血中Keap1-Nrf2/ARE通路功能的变化及临床意义

摘要

Objective:To study the changes and clinical significance of Keap1-Nrf2/ARE pathway function in peripheral blood of patients with ischemic stroke.Methods:The patients who were diagnosed with ischemic stroke in our hospital between June 2014 and March 2017 were selected as the stroke group of the study,and the healthy volunteers who received physical ex-amination during the same period were selected as the control group of the study.The peripheral blood was collected to deter-mine the expression of Keap1-Nrf2/ARE pathway molecules and downstream antioxidant enzymes,and serum was collected to determine the levels of Keap1-Nrf2/ARE pathway downstream antioxidant enzymes,nerve injury markers and oxidative stress products.Results:Keap1 mRNA expression in peripheral blood as well as HO1 and NQO1 levels in serum of stroke group was significantly lower than those of control group whereas Nrf2,ARE,HO1 and NQO1 mRNA expression were significantly higher than those of control group;serum β-actin,UCH-L1,NSE,S100B,GFAP,Hepc25,ROS,MDA,AOPP,ET-1 and TXB2 levels of stroke group were higher than those of control group,negatively correlated with Keap1 mRNA expression in peripheral blood and positively correlated with Nrf 2 and ARE mRNA expression in peripheral blood.Conclusion:The changes of Keap1-Nrf2/ARE pathway in peripheral blood of patients with ischemic stroke are closely related to the degree of nerve inju-ry and oxidative stress activation.%目的:研究缺血性脑卒中患者外周血中Keap1-Nrf2/ARE通路功能的变化及临床意义.方法:选择在本院诊断为缺血性脑卒中的患者作为研究的卒中组,同期在体检的健康志愿者作为研究的对照组.收集外周血并测定Keap1-Nrf2/ARE通路分子及下游抗氧化酶的表达量,收集血清并测定Keap1-Nrf2/ARE通路下游抗氧化酶、神经损害标志物、氧化应激产物的含量.结果:卒中组患者外周血中Keap1的mRNA表达量以及血清中HO1、NQO1显著低于对照组,Nrf2、ARE、HO1、NQO1的mRNA表达量显著高于对照组;卒中组患者血清中 β-actin、UCH-L1、NSE、S100B、GFAP、Hepc25、ROS、MDA、AOPP、ET-1、TXB2的含量高于对照组且与外周血中Keap1的mRNA表达量呈负相关,与外周血中Nrf2、ARE的mRNA表达量呈正相关.结论:缺血性脑卒中患者外周血中Keap1-Nrf2/ARE通路功能的变化与神经损伤及氧化应激反应激活的程度密切相关.