Correlation of Th1/Th2 balance in peripheral blood with cell apoptosis and invasion in decidual tissue in patients with recurrent spontaneous abortion

摘要

Objective:To study the correlation of Th1/Th2 balance in peripheral blood with cell apoptosis and invasion in decidual tissue in patients with recurrent spontaneous abortion (RSA). Methods: The subjects who were diagnosed with recurrent spontaneous abortion in Xiangyang Central Hospital between February 2015 and December 2017 were chosen as the RSA group, and the subjects who received early induced abortion in Xiangyang Central Hospital during the same period were chosen as the control group. The contents of Th1/Th2 cytokines and the expression of transcription factors in peripheral blood as well as the expression of apoptosis genes and invasion genes in decidual tissue were determined.Results: IL-2 and IFN-γ contents and T-bet expression in peripheral blood as well as CRT, Bax, Caspase-3, RECK and IL-1β mRNA expression in decidual tissue of RSA group were significantly higher than those of control group whereas IL-4 and IL-10 contents and GATA-3 expression in peripheral blood as well as HJURP, Bcl-2, HLA-G, MMP2, MMP9 and HOXA10 mRNA expression in decidual tissue were significantly lower than those of control group; IL-2 and IFN-γ contents as well as T-bet expression in peripheral blood of RSA group were negatively correlated with HJURP, Bcl-2, HLA-G, MMP2, MMP9 and HOXA10 mRNA expression in decidual tissue, and positively correlated with CRT, Bax, Caspase-3, RECK and IL-1β mRNA expression; IL-4 and IL-10 contents as well as GATA-3 expression in peripheral blood were positively correlated with HJURP, Bcl-2, HLA-G, MMP2, MMP9 and HOXA10 mRNA expression in decidual tissue, and negatively correlated with CRT, Bax, Caspase-3, RECK and IL-1β mRNA expression.Conclusion: The shift of Th1/Th2 balance to Th1 in peripheral blood of RSA patients may result in increased apoptosis and inhibited cell invasion in decidual tissues.