间歇低氧干预不影响实验性糖尿病大鼠骨骼肌SDH、LDH活性

摘要

目的：探讨间歇低氧干预对糖尿病大鼠骨骼肌糖代谢酶活性的影响。方法：采用长期高糖高脂膳食加小剂量链脲佐菌素（STZ）的方法建立糖尿病大鼠模型，将复制成功的模型大鼠随机分为模型对照组与低氧干预组。模型对照组在常氧环境下正常生活，低氧干预组大鼠每天在低氧帐篷内低氧暴露1h（O2浓度15．4±0．2％）、每周休息1d、共干预28d后36h取股四头肌测试琥珀酸脱氢酶（SDH）、乳酸脱氢酶（LDH）活性。结果：模型对照组大鼠股四头肌SDH活性显著低于正常对照组（P〈0．05），LDH活性高于正常对照组（P〉0．05），低氧干预组SDH、LDH活性与模型对照组比较均无明显差异（P〉0．05）。结论：实验性糖尿病大鼠骨骼肌SDH活性降低，有氧氧化供能能力下降，而LDH活性增强，无氧酵解能力提高；本实验所设计的间歇低氧干预模式对实验性糖尿病大鼠骨骼肌SDH、LDH活性均无明显积极作用。%Objective The purpose of this study was to investigate the effect of intermittent hypoxia on the SDH and LDH activities in skeletal muscle of diabetic rats. Methods The diabetic rat model was established by long-term high sugar and fat diet with low dose （30mg/kg body weight） of the streptozocin （STZ） by intraperitoneal injection only once. The diabetic rats were randomly divided into model control （MC） group and hypoxia interfering （HI） group. The MC rats were living in normal environment while the HI rats were exposed to hypoxia for one hour per day in hypoxic tent （oxygen concentration 15.4 ±0. 2 % ）, six days a week for four weeks. The activities of SDH and LDH in the quadriceps femoris rats were measured. Results Compared with the rats in normal control group, the SDH activity reduced significantly （ P 〈 0. 05 ） and LDH activity increased only slightly in model control group. No significant differences of the SDH and LDH activities were found between HI and MC group. Conclu- sion The study suggested that the aerobic oxidative capability reduced and glycolytic capability increased in skeletal muscle of diabetic rats. Hypoxia interfering for four weeks did not have effect on the SDH and LDH activities in skeletal muscle of diabetic rats.