目的：研究人骨髓间充质干细胞（ BMSCs）与3-羟基丁酸酯-3-羟基己酸酯共聚物（ PHBHHx）的生物相容性。方法：将PHBHHx（实验组）及明胶海绵（对照组）与第7代BMSCs复合培养7 d，通过光镜和扫描电镜分别观察BMSCs的生长及分布情况。结果：联合培养7 d，在显微镜下均可见两组材料周围有大量细胞生长，而材料内部及表面的细胞生长情况不能观察；电镜下见PHBHHx材料表面及孔隙内均有大量细胞附着，细胞相互连接融合成片，充满材料孔隙与表面，细胞表面可见大量的微绒毛，表示细胞生长状态良好，而明胶海绵上未见明显细胞生长。结论：PHBHHx具有良好的BMSCs相容性，可以作为一种新型的生物材料应用于组织工程。%Objective:To study the biocompatibility of bone marrow mesenchymal stem cells( BM-SCs)and 3-hydroxybutyrate-co-3-hydroxyhexanoate copolyesters( PHBHHx). Methods:The PHBH-Hx( experimental group)and gelatin sponge( control group)were cultured with BMSCs of the seventh generation for 7 days,and the growth and distribution condition of BMSCs were observed by light mi-croscopy and scanning electron microscopy. Results:After cultured for 7 days,under the microscope there were a large number of cells around the two materials,while inside and outside the material the cell growth cannot be observed;under electron microscope,attachment of a large number of cells were visible on the surface and in the pores of PHBHHx material,cells connected and were full of surface and pores,There are a lot of microvilli on cell surface,which showed cells in good condition,but no obvious cell growth were found in gelatin sponge. Conclusion:PHBHHx membrane has good BMSCs compatibility,and it can be used as a new biological materials in tissue engineering.
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