薏仁对小鼠细胞色素P4501A1酶活性硇影响

摘要

研究薏仁对细胞色素P4501A1酶活性的影响，为探讨其代谢机制及药用作用提供依据。昆明种小鼠各40只，按18．2g／kg·d^-1灌胃给药，生理盐水对照给药，分别在3周、6周和9周后采用分光光度法测定小鼠肝微粒体CYPlAl的活性。结果是薏仁能降低CYPlAl酶活性，且呈时间依赖性，抑制率在6．35％-46．50％之间；并对CYPlAl酶活性的抑制不存在性别差异。结论是薏仁可明显降低CYPlAl酶活性，且无性别差异。%Objective： To investigate the effects of Coix Lachrymal -jobion the activity of cyteehrome P4501A1 in mouse liver. Method： Coix Lachrymal -jobiwas administered intragastrally to male and female mice at doses of 18.2g/kg · d ^- 1. Controlled group was given by O. 9% NaC1 solution. Then enzymatic activity of CYP1A1 was detected by spectro- photometry at 3rd,6th or 9th weeks. Results： Enzymatic activity of CYP1A1 was inhibited byCoix Lachrymal-jobiranging from 6.35% to 46.50%, and the time course revealed that the inhibition reached plateau level at 9th week. There were not differences in activity of CYP1Al.between the sexes induced byCoix Lachrymal -jobi. Conclusions： Coix Lachrymal -jobicould inhibit the activity of CYP1A1, and no sex difference.