微波水解滞头废水中蛋白质试验

摘要

There are rich sericin protein and pupa protein in the frigon wastewater. Microwave digestion method is used to hydrolysis the protein extracted from frigon wastewater into amino acids mixture. Acid-catalyzed hydrolysis process conditions are studied and optimization, esp. the four factors and three levels of orthogonal test in a single-factor test. The results show that the effect on the conversion ratio of amino acids order is: feed ratio ＞ concentration of sulfuric acid ＞ hydrolysis time ＞ microwave power. The optimal hydrolysis conditions are as following: hydrolysis feed ratio is 1: 4; concentration of sulfuric acid is 2 mol/L; digestion time is 25 min;power is 400 W.%将滞头废水中蛋白质提取出来后,采用微波消解法将蛋白质水解成氨基酸混合液.研究了硫酸催化微波水解的工艺条件并对其进行优化,在单因素试验基础上采用四因素三水平正交试验.结果表明,对氨基酸转化率的影响依次为:料液比＞硫酸浓度＞水解时间＞微波功率;最佳水解工艺条件为:料液比为1:4,硫酸浓度2 mol/L,消解25 min,功率为400 W.