艾可清对猴艾滋病模型的治疗作用

摘要

[目的]应用猴艾滋病模型评价艾可清治疗艾滋病的效果.[方法]以猴免疫缺陷病毒SIVmac239感染12只恒河猴,复制猴艾滋病模型后分为3组:SIV对照组,艾可清高、低剂量组(剂量分别为445.5、148.5 mg·kg-1·d-1),感染l0周后开始给药.给药8周后停药观察8周.每4周采集外周静脉血,进行外周血CD4+、CD8+T淋巴细胞计数检测,分离血浆进行外周血SIV病毒载量检测.每8周行浅表淋巴结活检,进行苏木素—伊红(HE)染色,光镜下分析浅表淋巴结病理表现.[结果]艾可清高剂量组时猴艾滋病模型外周血CD4+T细胞具有一定升高作用,但停药后会下降；艾可清高剂量组血浆病毒载量均数在治疗第4、第8周时分别下降0.18806l0g和0.806 60 log,艾可清低剂量组治疗8周时则下降0.8142log;艾可清高、低剂量组均使浅表淋巴结病变保持稳定.[结论]艾可清对猴艾滋病模型外周血CD4+T淋巴细胞计数有一定的升高作用,时病毒栽量有一定的抑制作用,使浅表淋巴结病理变化保持稳定.%Objective To evaluate the therapeutic effect of Chinese herbal compound formula Aikeqing on simian immunodeficiency syndrome model. Methods Twelve rhesus monkeys were infected with SIVmac239 to induce simian immunodeficiency syndrome, and then were randomly divided into model group, high-dosage Aikeqing group (in the dosage of 445. 5 mg ? Kg"1 ? D"1) , and low-dosage Aikeqing group (in the dosage of 148. 5 mg "kg"1 ? D~' ). The medication was carried out 10 weeks after the infection and lasted 8 weeks. Observation was carried out for 8 weeks after suspension of the medication. Peripheral venous blood was sampled every 4 weeks for CD4 + and CD8 * T lymphocytes count. The viral load of simian immunodeficiency virus ( SIV) in peripheral plasma was also examined. Lymph node biopsy was conducted every 8 weeks, and the pathological features of the lymph node were observed under light microscope after HE staining. Results CD4+ T lymphocytes count in high-dosage Aikeqing group was increased during the medication, and then dropped down after suspension of the medication. The viral load of SIV decreased by 0. 188 06 log at the 4th week of medication and by 0. 806 60 log at the 8th week of medication in high-dosage Aikeqing group, and reduced by 0. 814 2 log at the 81 week of medication in low-dosage Aikeqing group. In Aikeqing groups, the pathologic features of superficial lymph nodes remained unchanged. Conclusion Aikeqing exerts certain effect on increasing peripheral CD4 * T lymphocytes count, inhibiting viral load, and stabilizing the pathologic changes of superficial lymph nodes in simian immunodeficiency syndrome model.