【目的】探讨抗癌防移片抑制4T1乳腺癌血管生成的机理。【方法】选用BALB/c小鼠建立4T1乳腺癌模型，随机分成空白对照组、模型组、环磷酰胺(CTX，剂量为0.04 g·kg-1·d-1)组和抗癌防移片组(剂量为5.2 g·kg-1·d-1)，分别给予药物或生理盐水，给药4周后处死小鼠，测量剥离瘤质量，计算剥离瘤质量抑制率以及计数肺转移结节数，并通过免疫组织化学染色法测定肿瘤微血管数与血管内皮细胞生长因子(vascular endothelial growth factor， VEGF)表达。【结果】与模型组比较，抗癌防移片组剥离瘤质量显著减轻（P<0.05），肺转移结节数目显著减少（P<0.05），且剥离瘤微血管数与VEGF表达有降低趋势。【结论】抗癌防移片可能通过下调VEGF、抑制肿瘤微血管生成从而抑制4T1小鼠乳腺癌生长与转移。%Objective To study the angiogenesis-inhibitory mechanism of Kang’ai Fangyi Tablets ( KFT) , a Chinese compound recipe with the action of inhibiting cancer metastasis, for 4T1 breast cancer. Methods BALB/c mice were divided into blank control group, model group, Cytoxan ( CTX, 0.04 g·kg-1·d-1) group, and KFT ( 5.2 g·kg-1·d-1) group. Mice model of 4T1 breast cancer was established. Except that the blank control group and model group were given the saline, the mice in the medication groups were given the corresponding medicine. After medication for 4 weeks, the mice were executed, and then we calculated the mass of tumor, the inhibition rate of tumor mass, and the number of lung metastatic nodules. The number of microvessel and expression of vascular endothelial growth factor (VEGF) were measured by immunohistochemical method. Results Compared with the model group, mice tumor mass was decreased ( P<0.05) , the number of pulmonary metastatic nodules was reduced ( P<0.05) , and the number of tumor microvessel and VEGF expression in the isolated tumor mass showed the decreasing trend in KFT group. Conclusion KFT can inhibit 4T1 breast cancer growth and metastasis by reducing the tumor microvessel formation and by affecting the expression of VEGF.
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