Objective: To investigate the effect of TNF-α and IL-1β on hepatic ischemia-reperfusion (IR) injury after sevoflurane preconditioning. Methods: Forty male adult SD rats were randomly divided into ischemia-reperfusion (IR) and sevoflurane ischemia-reperfusion (Sev) groups ( n = 20 for each). The animals were killed after hepatic IR injury. Serum ALT, AST, LDH, TNF-α and IL-1β were detected as liver damage markers. The histopathological changes of the liver were also observed. Results: ALT, AST and LDH were significantly inhihited in Sev ( P <0. 05). The injury score of the liver and the levels of IL-1β were lower in Sev than in IR groups ( P <0. 05). Conclusion: Sevoflurane preconditioning inhibits hepatic expression of IL-1β after IR injury. Sevoflurane preconditioning may have protective effect on hepatic IR injury through the inhibition of inflammatory cytokines.%目的:探讨七氟醚预处理对大鼠肝脏缺血再灌注损伤后肿瘤坏死因子(TNF-α)和白细胞介素1β(IL-1β)表达水平的影响.方法:将40只SD雄性大鼠随机分为两组,每组20只,即肝脏缺血再灌注损伤(IR)组,七氟醚预处理(Sev)组.分别于肝脏缺血前(T1)、肝脏再灌注后30 min(T2)、120 min(T3)各时点,经股动脉采血2 mL,血液离心取血清,测定大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)作为肝损害的标志,光镜检查组织病理学改变,检测大鼠血清TNF-α、IL-1β水平.结果:Sev组血清ALT、AST、LDH水平明显低于IR组(P<0.05),光镜下显示Sev组肝脏损伤程度小于IR组.Sev组IL-1β表达低于IR组(P<0.05),Sev组TNF-α的表达低于IR组,但差异无统计学意义(P>0.05).结论:七氟醚预处理可能通过抑制炎症细胞因子途径产生对肝脏缺血再灌注损伤起保护作用.
展开▼
