雌激素对前成骨细胞胰岛素受体底物-1的影响

摘要

目的:观察前成骨样细胞(MC3T3-E1细胞)分化过程中17 β-雌二醇对胰岛素受体底物-1(insulin receptor substrate-1,IRS-1)的影响.方法:应用10 mmol/L β-甘油磷酸钠和50 mg/L抗坏血酸诱导小鼠MC3T3-E1细胞分化成熟,分别在0,6,12,18,24,30 d收集细胞.17β-雌二醇干预,用半定量 RT-PCR和免疫印迹法检测干预组和对照组细胞IRS-1 mRNA和蛋白的表达,比较两组细胞IRS-1表达量的变化.结果:在MC3T3-E1细胞分化成熟过程中,IRS-1 mRNA和蛋白的表达在MC3T3-E1细胞分化成熟过程中逐渐增高.结论:IRS-1可能在17β-雌二醇诱导的骨转换过程中起着重要作用.%Objective: To observe the effect of estradiol on the expression profile of insulin receptor sub-strate-1 (IRS-1) during murine preosteoblastic MC3T3-E1 cells differentiation. Methods: MC3T3-E1 cell was cultured and underwent intervention of 1 7j3-estradial. Cells -were collected respectively in days 0, 6, 12, 18, 24 and 30. Semi-quantitative RT-PCR -was used to measure the expression of mRNA of IRS-1. The expression of the proteins of IRS-1 was detected by Western blotting. Results: IRS-1 mRNA and protein levels gradually increased during differentiation of MC3T3-E1 cells. Estradiol increased IRS-1 mRNA and protein expressions in the -whole time course. Conclusion: IRS-1 may play an important role in the process of estradiol inducing bone turnover.