二氢杨梅素对小鼠急性脑缺血缺氧的保护作用

摘要

Objective:To study the protective effect of dihydromyricetin (DMY) on acute cerebral hypoxiaischemia in mice.Methods:Male Kuming mice were randomly divided into control or sham group,high-and low-dose DMY group,ginaton group and model group.The mice in the high-and low-dose DMY group were administered 500 mg/kg and 250 mg/kg DMY respectively via intragastric gavage for 10 days.At 1 h after the last administration,the gasping time and gasping number of beheaded mice,survival time,oxygen consumption ratio in hypoxia tolerance mice,and survival time in bilateral carotid artery ligation (BCAO) mice were observed.Results:High-and low-dose DMY could significantly increased the gasping time and gasping number of beheaded mice,prolonged the oxygen consumption ratio of hypoxia tolerance mice and the survival time of BCAO mice.High-dos of DMY could also prolong the surviving time of hypoxia tolerance mice.Conclusion:DMY exerted a protective effect on acute cerebral hypoxia-ischemia in mice.%目的:研究二氢杨梅素(DMY)对小鼠急性脑缺血缺氧的保护作用.方法:将雄性昆明小鼠随机分为空白对照组(或假手术组)、DMY高剂量组、DMY低剂量组和金纳多组,必要时设模型组,每组30只.DMY高、低剂量组分别灌胃DMY500 mg/kg、250mg/kg,1次/d,共10d.末次给药1h后,采用小鼠断头实验,观察DMY对断头后小鼠喘气时间和喘气次数的影响;小鼠常压耐缺氧实验,观察DMY对小鼠存活时间、耗氧量和耗氧率的影响;小鼠结扎双侧颈总动脉实验,观察DMY对小鼠存活时间的影响.结果:与空白对照组或模型组比较,DMY高、低剂量可明显延长小鼠断头后喘气时间和喘气次数,降低常压缺氧小鼠耗氧率并延长双侧颈总动脉结扎小鼠的存活时间(P＜0.05或P＜0.01);此外,DMY高剂量还可延长常压缺氧小鼠存活时间(P＜0.05).结论:DMY对小鼠急性脑缺血缺氧具有一定的保护作用.