目的:探讨TLR9-MYD88-TRAF6-IRF5信号通路在系统性红斑狼疮(SLE)中的调控作用.方法:选取2016年5月至2017年10月广西医科大学第一附属医院收治的SLE患者38例为观察组,另选取同期在本院进行体检的19例健康志愿者作为对照组.采用酶联免疫吸附试验(ELISA)法检测两组血清中干扰素α(IFN-α)水平,采用实时荧光定量PCR(qPCR)法检测两组外周血单个核细胞(PBMC)Toll样受体9(TLR9)、接头蛋白髓系分化蛋白88 (MYD88)、肿瘤坏死因子相关受体因子6(TRAF6)、干扰素调节因子5(IRF5) mRNA相对表达量.结果:观察组血清IFN-α水平及PBMC中TLR9、MYD88、TRAF6、IRF5 mRNA水平均较对照组明显升高(均P＜0.05).在观察组中,TLR9的表达量与MYD88、TRAF6、IRF5、IFN-α的表达量及系统性红斑狼疮疾病活动指数(SLEDAI)均呈正相关关系(P＜0.05),与补体C3、补体C4的表达量呈负相关关系(P＜0.05);MYD88、TRAF6、IRF5的表达量均与SLEDAI呈正相关关系(P＜0.05).结论:TLR9-MYD88-TRAF6-IRF5信号通路可能参与SLE发病的过程,该信号传导途径的激活可能与疾病活动相关.%Objective:To study the regulation of TLR9-MYD88-TRAF6-IRF5 signaling pathway in systemic lupus erythematosus (SLE).Methods:38 SLE patients treated in our hospital from May 2016 to October 2017 were selected as an observation group,19 healthy subjects were enrolled as a control group in the same period.The level of interferon (IFN)-α in serum was detected by enzyme linked immunosorbent assay (ELISA).The mRNA expressions of Toll-like receptor 9 (TLR9),myeloid differentiation primary-response protein 88 (MYD88),tumor necrosis factor receptor-associated factor 6 (TRAF6) and interferon regulatory factor 5 (IRF5) in peripheral blood mononuclear cells (PBMC) were analyzed by qPCR.Results:The IFN-α level in serum and TLR9,MYD88,TRAF6 and IRF5 mRNA levels in PBMC were significantly higher in the observation group,compared with the control group (P ＜0.05).The TLR9 expression was positively correlated with the MYD88,TRAF6,IRF5 and IFN-α expressions as well as the SLE disease activity index,and was negatively related to complement C3 and complement C4 (P ＜ 0.05).Conclusion:The TLR9-MyD88-TRAF6-IRF5 signaling pathway might play a role in the pathogenesis of SLE,the activation of which could result in active SLE.
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