C型产气荚膜梭菌β毒素蛋白结构及抗原表位预测

摘要

The protein structure and epitope ofβtoxin of Clostridium perfringens type C were predic-ted and analyzed by DNASTAR and I-Tasser software.The prediction results of hydrophilicity,surface possibilities,antigen index and secondary structure showed that 2~4,7~16,23~26,36~40,55~58,72~75,172~179,190~193,196~203,211~214,246~249 existed probably antigen epitope of B cell.This re-sults combining tertiary structure showed that 190~193 and 211~214 probably would become the antigen epitope of B cell.%利用DNASTAR、I-Tasser软件对C型产气荚膜梭菌β毒素的蛋白结构、抗原表位进行预测与分析。综合β毒素蛋白亲水性、表面可能性、抗原指数、二级结构预测结果显示,B细胞抗原表位可能位于肽链2~4、7~16、23~26、36~40、55~58、72~75、172~179、190~193、196~203、211~214、246~249位氨基酸区域；结合三级结构预测结果显示,190~193、211~214成为表位的可能性较大。