感染茶假眼小绿叶蝉的金龟子绿僵菌菌株筛选

摘要

采用喷雾法测定8株金龟子绿僵菌菌株对假眼小绿叶蝉的致病力。结果表明：以浓度（1±0．5）×107孢子· mL-1的绿僵菌孢子悬液接种12 d后，各菌株对假眼小绿叶蝉均有一定的致病力，校正死亡率和僵虫率分别为（43．08±0．76）％-（76．92±1．72）％和（40．67±1．25）％-（76．67±1．55）％，LT50为6．76-11．48 d。其中，Ma1775菌株对假眼小绿叶蝉致病力最强，校正死亡率和僵虫率分别为（76．92±1．72）％和（76．67±1．55）％，LT50为6．76 d，6 d的LC50为4．1563×107孢子· mL-1。通过时间-剂量-死亡率模型参数估算，Ma1775菌株对该虫致死效应较强的时间段为3-5 d，该菌株在假眼小绿叶蝉的生物防治中有很大的应用潜力。%The virulence of 8 strains of Metarhizium anisopliae against Empoasca vitis was determined by spraying.The results showed that various strains of M.anisopliae had certain virulence against E.vitis after inoculation with (1 ±0.5) ×107 spores· mL-1 for 12 days.The ranges of the corrected mortality and cadaver rate were from (43.08 ±0.76)% to (76.92 ±1.72)%, and from (40.67 ±1.25)% to (76.67 ±1.55)%, respectively.The median lethal time ( LT50 ) was from 6.76 days to 11.48 days.When various strains were compared, Ma1775 strain exhibited the most effective virulence against E.vitis, causing a corrected mortality of (76.92 ±1.72)%, the cadaver rate of (76.67 ±1.55)% and the median lethal time ( LT50 ) of 6.76 days.The LC50 of Ma1775 strain to E.vitis on the 6th day was 4.156 3 ×107 spores· mL-1 .A time-dose-mortality model was employed to estimate parameters of time and dose, which showed that Ma1775 strain had a high lethal effect on E.vitis during between 3 and 5 days in the treatment, so Ma1775 strain has been proved to be a great potential application in biological control to E.vitis.