龙眼胚性愈伤组织长期继代培养及其染色体数目变异

摘要

Friable embryogenic calli induced and then selected from the culture of longan (Dimocarpus longanLour. ) immature embryos maintained strong capacity of somatic embryogenesis after having been subculturedfor over 6 years. The cytological examinations showed that there were some cell variations with thechromosome number 2n＝3x＝ 45 or 2n＝ 4x＝ 60 or others in some cell clusters of callus lines, which was ofgreat value for obtaining cell variants in vitro in longan.%摘要：从龙眼幼胚培养诱导并筛选出的松散型胚性愈伤组织，在继代培养基上已培养6 a以上，所保持的若干愈伤组织仍具有强烈的体细胞胚胎发生能力.经细胞学检查，其中个别愈伤组织系的部分愈伤组织团发现有2n=3x=45或2n=4x=60等染色体数目的变异细胞，这对于离体筛选龙眼细胞突变体很有价值.