番木瓜胚性愈伤组织的诱导及体胚发生

摘要

This test regards immature embryos,leaves and cotyledons of papaya(Carica papaya L.)as explants,studying and exploring the effect of hormones, attachment, developing methods and conditions on the papaya embryogenic calli and somatic embryos induction.The experimental results showed that immature embryo was suit to somatic embryogennesis.The best medium for callus induction was modified MS+10 mg .L-1 2,4-D+5 mg .L-1 NAA+2 mg .L-1 KT+0.5 mg .L-1 BA,the most suitable medium for callus proliferation was modified MS+10 mg . L-1 2,4-D+2.5mg .L-1 NAA+1 mg .L-1 KT+0.5 mg . L-1 BA, and the best medium of somatic embryogenesis was modified MS + 10 mg . L-1 2,4-D +2 mg . L-1 KT. Embryogenic calli inducion from papaya coty1edon was difficult, calli induction from leaves or hypocotyl was easy, but somatic embryogenesis was very difficult.%以番木瓜的幼胚、下胚轴、叶片和子叶作为外植体,研究不同的激素配比、附加物以及培养方式和条件对胚性愈伤组织和体胚的诱导效果.结果表明,幼胚是胚性愈伤组织和体胚发生的好材料.幼胚最佳诱导培养基为:改良MS+10 mg·L-12,4-D+5 mg·L-1NAA+2 mg·L-1KT+0.5 mg·L-1 BA;最适合的增殖培养基为:改良MS+10 mg·L-12,4-D+2.5mg.L-1 NAA+1 mg·L-1 KT+0.5 mg·L-1 BA;最佳体胚诱导培养基为:改良MS+10 mg·L-1 2,4-D+2 mg·L-1 KT;子叶较难诱导愈伤组织,叶片和下胚轴愈伤组织的诱导率较高,但愈伤组织质量较差,体胚发生率极低.