Effect of plant growth regulators on direct somatic embryogenesis in camphor tree (Cinnamomum camphora L. ) from immature zygotic embryos and embryogenic calli induction

摘要

A description of a successful direct somatic embryogenesis induction from immature zygotic embryos of a camphor tree (Cinnamomum camphora L. ) is presented. After a subculture of 2-3 years,embryogenic calli could be derived from primary somaticembryos. Immature zygotic embryos were cultured on a Murashige and Skoog (MS)basal medium supplemented with a range of combinations of cytokinins (BA) and auxins(2,4-D or NAA) for somatic embryo induction. Primary somatic embryos could be induced direly in almost all PGR combinations. A positive effect of 2,4-D On somatic embryo genesis from immature zygotic embryos of camphor tree was obtained. BA at appropriate concentrations (<5mg·L-1)had an effect similar to 2,4-D,whereas high concentrations (>5mg·L-1) of BA had the effect of restraining somatic embryo induction. NAA had a less positive effect on somatic embryogenesis than 2,4-D.