毛竹生长素响应因子基因家族的鉴定及其在竹笋发育中的表达

摘要

To investigate the role of auxin response factor( ARF) in regulating shoot development of moso bamboo, 39 PhARF genes from moso bamboo genome were identified via BlastP analysis. Bioinformation analysis revealed that PhARF genes were conservative among species. Dynamic expression pattern of PhARF family was further examined by RT-PCR during young bamboo development process. The results showed that PhARF18 and 11-2 maybe were the main ARF transcription factors in bud germination and young bamboo formation stage, respectively, with PhARF6-2 making difference in both stages.%以已知拟南芥和水稻生长素响应因子( ARF)蛋白质序列对毛竹基因组数据库进行比对分析显示，毛竹基因组编码39个ARF转录因子；通过生物信息学软件对毛竹ARF基因家族进行分析显示，毛竹ARF基因家族具有进化上的保守性；采用RT-PCR技术研究毛竹竹笋形成过程中ARF基因家族的动态变化，结果显示，在竹笋形成过程中ARF18和11-2基因分别在笋芽萌动和笋体形成过程中发挥调控作用，而ARF6-2基因参与竹笋发育的各个阶段。