人前列腺癌中P21CIP1/WAF1, Rb的表达意义

摘要

AIM　To research the expression and correlation of P21CIP1/WAF1 and Rb in human prostate cancer, for the purpose of illustrating their relations to the pathological grading and clinical phases. METHODS　We tested the 36 samples of prostate cancer by applying the immunohistochemical SABC method and observed the expression and correlation of P21CIP1/WAF1 and Rb in human prostate cancer, and applied this method to the study of the clinical phases. RESULTS　In the above mentioned 36 samples, the immunohistochemistry positive of P21CIP1/WAF1 and Rb showed the color of brown or dark brown, which were in cytoplasm and nucleus. Among them, 19 cases is P21CIP1/WAF1 positive, rated 52.8%, and 17 is Rb positive, rated 47.2%. Statistically the significant differences of P21CIP1/WAF1 and Rb were found in different pathological grading and clinical phases but no significant correlation was found between p21CIP1/WAF1 and Rb respectively. CONCLUSION　Experiment shows a negative correlation between the expression of P21CIP1/WAF1 and Rb and the pathological grading and clinical phases. The pathogenic mechanism of the prostate cancer is related to the abnormal path for P21CIP1/WAF1 and Rb.%目的研究P21CIP1/WAF1及Rb在人前列腺癌标本中的表达以及两者的相关性，以阐述它们与前列腺癌的病理分级及临床分期的关系. 方法应用免疫组化SABC法染色技术对36例前列腺癌标本进行检测，观察P21CIP1/WAF1及Rb在前列腺癌各病理分级，临床分期中的表达及相关性. 结果在36例人前列腺癌标本中，P21CIP1/WAF1和Rb免疫组化阳性染色为棕黄色或棕褐色，分别定位于细胞质和细胞核，其中P21CIP1/WAF1阳性19例，阳性率52.8%. Rb阳性17例，阳性率47.2%. 在不同病理分级、临床分期之间差异均有显著性，但实验结果未发现P21CIP1/WAF1与Rb有显著相关性. 结论 P21CIP1/WAF1, Rb的表达与前列腺癌组织的病理学分级、临床分期呈负相关性，在前列腺癌分子发病机制中可能涉及到P21CIP1/WAF1, Rb调节通道的异常.