过敏性休克大鼠血嗜碱性粒细胞CD63的表达

摘要

目的 通过流式细胞仪分析CD63的表达来确定嗜碱性粒细胞活化情况,探讨其在过敏性休克诊断中的应用价值.方法 16只大鼠随机分为对照组和实验组,采用卵蛋白注射建立过敏性休克大鼠动物模型,以CD63、CD45、CD203c抗体组合,应用流式细胞仪检测大鼠血嗜碱性粒细胞CD63的活化情况,并采用免疫荧光法对大鼠肺组织CD63进行免疫荧光染色. 结果 (1)以CD45和CD203c联合设门,可以得到纯的嗜碱性粒细胞.(2)实验组CD63的表达率为(17.34±2.04)％,对照组CD63的表达率为(1.52±0.35)％,差异具有统计学意义(P＜0.01).(3)与对照组相比,实验组大鼠肺组织中嗜碱性粒细胞CD63的表达增多.结论 流式细胞仪检测CD63的表达有望作为过敏反应体内试验的补充,具有较好的临床应用价值.%Objective To explore the value of flow cytometry in anaphylactic shock diagnosis by CD63 expression being detected using flow cytometry to conform the activation of basophils. Methods Sixteen rats were randomly divided into two groups: control group and anaphylactic shock group. The model of anaphylactic shock rat with ovalbumin injection was established. CD63, CD45 and CD203c antibody combination, flow cytometry was employed to detected blood basophil CD63 expression. Immunofluorescence method was employed to observe the CD63 immunofluorescence staining in the rat lung tissue. Results (1) Pure basophils were obtained by CD45 and CD203c gating. (2) The percentages of basophils CD63 were (17.34± 2.04)% and (1.52+0.35)% in the experimental and control group, respectively. The differences between two groups were statistically significant(P<0.01). (3) Compared with the control group, the expression of CD63 in basophils increased in anaphylactic shock lung tissue. Conclusion The detection of CD63 by flow cytometry could be the supplement of vivo allergic reactions and have good clinical value.