小鼠皮肤烫伤模型的建立

摘要

Objective To establish the model of skin scald in mice for the study of skin thermal injuries. Methods After anaesthetization mice were scalded in a 1 cm-diameter circle area on the central dorsum by boiling water at contact times: 10s or 25s. The mice were sacrificed at 1, 3, S, 7 and 10 days after scald. The skin samples were collected and analyzed by gross and histopathological examinations. Results Deep Ⅱ degree thermal injury involving full-thickness skin was observed in the 10 s scald group. Ⅲ degree thermal injury involving full-thickness skin and the dorsal skeletal muscle was observed in the 25 s scald group. Conclusion A mouse skin scald model is established which is stable and can be used on the skin thermal injury in future research.%目的 建立用于热损伤研究的小鼠皮肤烫伤动物模型. 方法 小鼠麻醉后,使用沸水在其背部烫出直径1cm的圆形烫伤区,沸水接触时间分别为10s和25s.烫伤后1、3、5、7和10d处死动物.对不同损伤时间点皮肤取材,进行肉眼和组织学检查. 结果 10s烫伤组,烫伤累及全层皮肤,程度为深Ⅱ°；25 s烫伤组烫伤累及全层皮肤及背部肌肉,程度为Ⅲ°.结论 该模型采用简单的方法成功制作出较为稳定的皮肤烫伤模型,为以后的皮肤热损伤研究奠定了一定的基础.