15个常染色体和10个Y-STR基因座复合扩增试剂盒的研发

摘要

Objective To establisha multiplex STR genotyping m ethod for autosom al STR and Y-STR loci in forensic biological practice. Methods W idely used autosom al STR loci and Y-STR loci w ere se-lected. A set of PC R prim ers w as designed, and a 5-dye fluorescent labeled STR multiplex PC R reagent kit w as developed. Results A kit w as developed w hich can sim ultaneously detect 15 autosom al STR loci, 10 Y-STR loci, and an Amelogenin. Conclusion The 15 autosom al STR plus 10 Y-STR kit in com bination w ith capillary electrophoresis m ethod w as used to STR genotyping w ith accurate and reli-able results. The new one-step testing kit can potentially be w idely used in forensic cases and D N A databank in the future.%目的：建立法医物证学常染色体和Y染色体综合检测的方法。方法选择常用的常染色体STR基因座和Y染色体STR基因座，设计复合扩增引物，形成五色荧光标记复合扩增试剂盒。结果开发出一个五色荧光标记复合扩增试剂盒，可同时对15个常染色体STR基因座、1个性别基因座和10个Y染色体STR基因座进行分型检测。结论15个常染色体STR加10个Y染色体STR检测试剂盒结合毛细管电泳凝胶进行STR分型，结果准确可靠，在法医学案件检验及数据库建设等方面有良好的应用前景。