HIF-1α、VEGF-A在心律失常大鼠心肌组织中的变化

摘要

目的 观察大鼠心律失常后缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)和心肌血管内皮生长因子-A(vascular endothelial growth factor-A,VEGF-A)的表达变化,探讨心律失常与冠状动脉供血不足引起的急性心肌缺血中两指标表达规律的不同.方法 利用CaCl2诱导大鼠心律失常,应用免疫组织化学染色、Western印迹法和实时荧光定量PCR检测大鼠心律失常后6 h内HIF-1α和VEGF-A的表达及变化情况.结果 心律失常致死的大鼠心肌组织中HIF-1α及VEGF-A呈弥漫性表达,在心律失常发生的早期两者表达均呈增加随后下降;心律失常发生时即产生广泛的心肌缺血且范围不随时间增大.结论HIF-1α和VEGF-A在心律失常大鼠心肌组织中均有表达,可为致死性心律失常和冠状动脉供血不足引起的急性心肌缺血的鉴别诊断提供依据.%Objective T o observe the expression changes of hypoxia inducible factor-1α (H IF-1α) and vascular endothelial grow th factor-A (V E G F-A ) in rats w ith arrhythm ias, and to explore the differences of the expression pattern in the tw o indicators of acute m yocardial ischem ia caused by arrhythm ias and coronary insufficiency. Methods T he arrhythm ia w as induced by C aC l2, and the expression changes of H IF-1α and V E G F-A w ere detected by im m unohistochem istry, W estern blotting and real-tim e PC R w ithin 6 h after the arrhythm ia in rats. Results T he expression of H IF-1α and V E G F-A show ed diffuse in the m yocardial tissue of rats died from arrhythm ias. B oth of them increased in the early arrhythm ia, then decreased. E xtensive m yocardial ischem ia happened at the beginning of arrhythm ia occurrence and its range didn't expand w ith tim e. Conclusion T he expressions of H IF-1α and V E G F-A in m yocardium of the rats w ith arrhythm ia can provide evidence for the differential diagnosis of acute m yocardial is-chem ia caused by fatal arrhythm ia and coronary insufficiency.