高左旋多巴合成活性的重组大肠杆菌培养条件优化

摘要

对重组大肠杆菌进行培养条件的研究，得到细胞合成L-DOPA最佳培养条件为：L-tyrosine 0.2%、KH2PO4　0.2%、MgSO4*7H2O　0.1%、FeSO4*7H2O　0.001%、ZnSO4 0.001%、磷酸吡哆醛　0.005%、牛肉膏　0.5%、蛋白胨　2%、DL-Alanine　0.1%、温度37 ℃、pH值7.0、培养时间16 h、摇瓶转速150 r/min.%Culture conditions for recombinant E.coli containing high tyrosine phenol lyase were studied. Optimum condition of enzyme formation was cells cultured at 37 ℃for 16hours in the medium containing 0.2% L-tyrosine, 0.2%KH2PO4, 0.1%　MgSO4*7H2O, 0.001%　FeSO4*7H2O, 0.001%　ZnSO4*7H2O, 0.005%　pyridoxal phosphate, 0.5%　meat extract, 2%　polypeptone, and 0.1%　DL-Alanine. Initial pH and rotation rate were 7.0 and 150　r/min respectively.