为了充分利用提取木脂素后的五味子废渣,提高其附加值,采用水提醇沉法提取五味子粗多糖,经聚酰胺柱层析及Sephacryl S-300 HR凝胶柱层析对其进行纯化,将得到的级分SCP-0采用Sephacryl S-300 HR凝胶柱层析法及凝胶渗透色谱法进行纯度鉴定;然后对SCP-0进行体外清除自由基活性测定.结果显示,其对·OH具有较好的清除效果,IC50为14.5 mg/mL;对DPPH·具有较好的清除效果,IC50为2.1 mg/mL;但对·O2-无明显清除效果;具有一定的抗油脂氧化能力,在低自由基浓度水平下效果更明显.%The aim of this study is to find a way to reuse Schisandra chinensis fruits wastes after lignans extration. Polysaccharide tabled as SCP -0 was separated by hot -water extraction and ethanol precipitation, and then purified by polyamide column chromatography and Sephacryl S-300 HR column chromatography. After purity identification, the antioxidant activities of SCP-0 were investigated and the results showed a noticeable scavenging activity against hydroxyl radical and DPPH with IC50 of 14.5 mg/mL and 2.1 mg/mL respectively. However, superoxide anion scavenging activity of SCP -0 was hardly observed and certain ability of restraint on lard oxidation was observed.
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