蛹虫草Cordyceps militaris JN168产虫草素液态发酵条件的优化

摘要

利用单因素筛选和响应面法对蛹虫草Cordyceps militaris JN168产虫草素的液态发酵培养基进行优化,以确定蛹虫草产虫草素的最佳发酵培养基配方.结果表明,蛹虫草产虫草素的最佳碳源为葡萄糖,最适质量浓度为40 g/L;最佳氮源为牛肉膏,最适质量浓度15 g/L;加入的无机盐及其添加量分别为MgSO4 0.76 g/L,K2HPO4 0.63 g/L,CaCl2 0.66 g/L,Na2HPO4 0.67 g/L.优化后发酵液中虫草素质量浓度达到633.47 mg/L,是优化前的6倍.%The components for cordycepin production by Cordyceps militaris JN168 were optimized with single factor experiment and response surface methodology. The purpose of this study is to determine the best fermentation medium for cordycepin production by Cordyceps militaris JN168. Results showed that glucose and beef extract are the appropriate carbon and nitrogen sources with an optimal concentration of 40 g/L and 15 g/L, respectively. And adding inorganic salt is MgSO4, K2HPO4,CaCl2 and Na2HPO4 with an optimal concentration of 0.76 g/L,0.63 g/L,0.66 g/L and 0.67 g/L respectively. The cordycepin concentration in the fermentation broth after optimization is 633.47 mg/L, which is 6 times before the optimization.