雨生红球藻是虾青素的主要来源,为探明黄腐酸(FA)对雨生红球藻Haematococcus pluvialis LUGU的影响,将不同质量浓度的FA添加至对数生长期的藻液中置于胁迫条件下培养,并分别对细胞生物量浓度、虾青素积累质量浓度以及番茄红素β-环化酶(lcy)基因表达量进行了测定.结果表明,黄腐酸质量浓度为10 mg/L,藻细胞的生物量产率达到最大80.28 mg/(L·d),比对照组提高5.44％;而虾青素最大质量浓度为20.82 mg/L,是在FA质量浓度为5 mg/L时测得,比对照组提高了86.89％.RT-PCR分析显示,虾青素合成的关键基因lcy的表达受FA的影响,当添加5 mg/L和10 mg/L的黄腐酸时lcy基因最大的表达量分别为对照的1.2倍和0.7倍.实验证明FA诱导下的雨生红球藻虾青素的积累含量和lcy基因表达量呈正相关.%To explore the effect of Fulvic acid (FA) on Haematococcus pluvialis,different concentration gradient of Fulvic acid (FA) was added in the culture of Haematococcus pluvialis LUGU.Meanwhile,cells growth,astaxanthin accumulation and the transcript levels expression kinetics of lcy gene was studied during induced time.The results show that the suitable concentration of FA not only promote the algae growth (when dealt with 10 mg L-1 FA concentration,the algae biomass production reach the maximum 80.28 mg L-1d-1,increased by 5.44％ than that of the control group),but also greatly improve the astaxanthin content (the astaxanthin content reach 20.82 mg L-1 when dealt with 5 mg L-1 FA concentration,is 86.89％ higher than the control group).RT-PCR analysis shows that the transcriptional levels of lcy induced by 5 mg L-1 and 10 mg L-1 FA is 1.2 and 0.7 times than that of control,respectively.The correlation analysis indicate that,the accumulation of astaxanthin content and the transcriptional levels of lcy gene inducted by FA is positively correlated.
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