液相色谱法与氨基酸分析仪法测定人乳中水解氨基酸的比较研究

摘要

目的：研究氨基酸自动分析仪法和液相色谱法测定人乳中水解氨基酸含量的比较。方法采用液相色谱柱前衍生和氨基酸分析仪测定人乳中水解氨基酸，液相色谱法选用 C18+柱和 AQC 进行衍生，氨基酸分析仪采用阳离子交换柱和柱后茚三酮衍生。结果两种方法的方法学参数无明显差异，所测结果差异均在4.65%以内。结论两种方法均可采用，可作为互相验证的方法。%Objective Comparison the methods of amino acid analyzer (AAA) and ultra-performance liq-uid chromatography (UPLC) for determination of hydrolysis amino acids in human milk. Methods Hydroly-sis amino acids were analysed with C18+column and AQC precolumn derivation by UPLC method;and ion ex-change chromatography column and ninhydrinpostcolumn derivation by AAA method. Results Two methods provided similar validation results. The difference of analysis result using two methods was below 4.65%. Conclusion Both methods can be used for the quantitation of hydrolysis amino acids.