The molt cycle of the Chinese mitten crab Eriocheir sinensis was staged by the method of Darch. We then measured the three digestive enzymes in hepatopancreas throughout the molt cycle. Based on the setogenesis and the morphological changes to the epidermis of the third maxilliped, we identified four molting periods (post-molt, intermolt, premolt and ecdysis). In addition, the premolt period was further divided into three substages (D0, D1, and D3-4). Both amylase and tryptase activities increased in the hepatopancreas during the A-B stages, peaking in the early premolt D0 stage (P＜0.05), and then gradually decreasing in the later premolt D3-4 stage (.P＜0.05). There were no obvious changes in peptase activity during the majority of the molt cycle, though levels were significantly lower (P＜0.05) in the later premolt D3-4 stage. In summary, we identified five periods (C, D0, D1, D3-4 A-B)in the molt cycle of E. sinensis during which the activities of amylase, trypsase, and peptase changed periodically.%采用Darch蜕皮周期分期方法,对中华绒螯蟹(Eriocheir sinensis)的蜕皮周期进行了划分,并初步探讨了中华绒螯蟹蜕皮过程中肝胰腺消化酶活性变化.根据第三颚足末端刚毛和表皮的形态学特征,可将中华绒螯蟹的蜕皮周期分为蜕皮后期(A-B期)、蜕皮间期(C期)、蜕皮前期(D期)和蜕皮期(E期),其中D期可进一步分为D0、D1、D3-43个亚期.在蜕皮过程中,肝胰腺淀粉酶和胰蛋白酶活性在蜕皮后A-B期开始升高,于蜕皮前早期D0阶段达最大(P＜0.05),随后又逐渐降低,至蜕皮前晚期D3-4阶段降至最低(P＜0.05)；胃蛋白酶活力在蜕皮周期中于蜕皮前晚期D3-4阶段显著降低(P＜0.05),其他阶段变化不明显(P＞0.05).结论认为,中华绒螯蟹蜕皮过程可分为C、D0、D1、D3-4、A-B 5个时期,蜕皮过程中肝胰腺淀粉酶、胰蛋白酶和胃蛋白酶活性发生周期性变化.
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