本研究探讨干扰素α对慢性髓系白血病(CML)来源的树突状细胞(DC)表达Fas/FasL的影响.在CML-DCs的培养液中除加入SCF,GM-CSF,TNF-α及IL-4外,还加入IFN-α.培养10-14天,除了鉴定细胞免疫表型和Ph1染色体比例外,还应用流式细胞仪检测细胞表达Fas/FasL比例,用PI染色分析细胞凋亡,ELISA法检测上清液sFas含量.结果表明:加入IFN-α后,CML-DC共刺激分子的表达显著改善,Ph1(+)细胞比例随IFN-α浓度增加而减低;培养细胞Fas的表达上调,sFas含量却下降,FasL表达阴性,细胞凋亡比例增加.结论:IFN-α在改善CML-DC表型同时,可通过Fas途径促进Ph1(+)细胞凋亡,使Ph1'(-)细胞数量相对增加.%The study was aimed to investigate the influence of interferon α (IFN-α) on the expressions of Fas and Fas ligand (FasL) in dendritic cells (DCs) from patients with chronic myeloid leukemia (CML). In addition to adding stem cell factor(SCF), granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor α (TNF-α) and interleukin 4 (IL-4), the IFN-α was added to the serum-free medium for DCs. After culturing for 10-14 days,cell phenotype and percentage of Ph1 chromosome were detected by different methods. The expression of Fas or FasL on CML-DCs and cell cycle of DCs labeled with propidium iodine (PI) were measured by flow cytometry. The concentra-tion of sFas in supernatants was analyzed by enzyme-linked immunosorbent assay (ELISA). The results indicated thatthe expression of co-stimulatory molecules were improved significantly while the percentages of Ph1 positive cells de-creased. The level of Fas on cells was up-regulated and the concentration of sFas decreased. However, the expression of FasL was negative. The ratio of apoptosis rose gradually while the concentration of IFN-α increased. It is concluded that IFN-α can accelerate the apoptosis of Ph1 positive cells through Fas/FasL pathway, so the number of Ph1 negative cells increases relatively.
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