c-myc、c-myb基因在白血病骨髓基质细胞中的表达及相关性研究

摘要

This study was purposed to investigate the expression level of oncogene c-myc and c-myb in leukemic cells and leukemic bone marrow stromal cells (BMSC) and the correlation with each other. The expression levels of c-myc and c-myb in those cells were examined semi-quantitatively by reverse transcription polymerase chain reaction (RT-PCR). Flow cytometry analysis was used to detect the membrane surface antigens of leukemic cells and BMSC. The karyotype was analyzed by G-banding techniques. The results showed that (1) c-myc and c-myb gene were expressed in the normal control group, the leukemic cells and BMSC of patients group. The mean expression levels of c-myb mRNA and c-myc mRNA in abnormal chromosomal leukemic cells were 1. 03 ±0.48 and 1. 15 ± 0. 38 respectively, which were significantly higher than those in control group (P <0.05). In the abnormal karyotype stromal cells, the mean expression level of c-myb mRNA and c-myc mRNA were 2.08 ± 0.82 and 1.46 ± 0. 29 respectively (P < 0. 05).rn(2) The expression level of c-myc and c-myb mRNA were closely associated with patients' platelet counts (P < 0.05).rn(3) The expression of c-myc mRNA linearly correlated with the expression of c-myb mRNA in different prognostic groups. (4) In acute leukemic cells and BMSC, c-myc expression positively correlated with c-myb expression. (5) The expression level of c-myc in leukemic cells correlated with the expression levels of c-myc and c-myb in BMSC, respectively. It is concluded that the reduction of c-myc or c-myb expression levels may be a therapeutic regimen for leukemia.%本研究检测分析c-myc、c-myb原癌基因在白血病细胞和骨髓基质细胞(BMSC)中的表达及其相关性.用逆转录聚合酶链反应( RT- PCR)技术定量分析原癌基因c-myc、c -myb的表达水平,通过流式细胞术分析白血病细胞和BMSC的膜表面抗原,同时采用染色体显带技术分析细胞核型.结果表明:①c-myc、c-myb基因在白血病细胞及其BMSC中均有一定的表达,尤其是在染色体异常的白血病细胞中高表达,其均值分别为1.15±0.38和1.03±0.48,与对照组比较均具有统计学差异(P＜0.05)；在染色体异常的BMSC中其均值分别为2.08±0.82(P ＜0.05)和1.46±0.29(P ＜0.05)；②在白血病细胞和BMSC中,c-myc mRNA、c-myb mRNA的表达水平均与血小板计数具有相关性；③在不同预后组中,c-myc mRNA、c-myb mRNA表达呈线性相关；④在白血病细胞及急性组BMSC中原癌基因c-myc、c-myb的表达存在正相关；⑤白血病细胞中c-myc的表达量分别与BMSC中c-myc、c-myb的表达量有相关性.结论:在白血病中降低c-myc或c-myb原癌基因的表达水平可能成为治疗白血病的一种治疗方案.